DNA analysis of the thermophilic Campylobacter by pulsed-field gel electrophoresis.
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چکیده
منابع مشابه
Campylobacter Epidemiology – Insights from Subtyping by Pulsed-Field Gel Electrophoresis
Höök, H. 2005. Campylobacter epidemiology – insights from subtyping by pulsed-field gel electrophoresis. Doctoral thesis. ISSN 1652-6880, ISBN 91-576-6988-0 Campylobacter jejuni and Campylobacter coli are frequent worldwide causes of food-borne gastroenteritis. Poultry is regarded as the most important infection source. In temperate countries, a marked seasonal variation of human campylobacteri...
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Mobility of supercoiled (form I) and nicked circular (form II) plasmid DNAs was determined on two major forms of pulsed-field electrophoresis, CHEF and OFAGE. Plasmids with molecular lengths ranging from 2.30 to 17.8 kilobase pairs (kb) were used with Saccharomyces cerevisiae chromosomes as standards. Agarose gel concentrations were varied from 0.3 to 2.0 percent, with higher percentage gels re...
متن کاملPulsed-field gel electrophoresis.
DNA molecules in an agarose matrix elongate and migrate toward the anode when exposed to an electric field. An agarose matrix represents a highly irregular network with pores of various dimensions, large open areas, and regions with different densities. During electrophoresis, migration will be retarded by obstructions that prevent sieving by size and result in "end-on" migration as if through ...
متن کاملGenetic analysis of Pseudomonas aeruginosa by pulsed field gel electrophoresis.
We identified the serotypes and genomes patterns of 100 strains of Pseudomonas aeruginosa (P. aeruginosa) that had been isolated from patients who were admitted to the hospital of the Fukui Medical School between 1992 and 1995. A monoclonal diagnostic kit was used to identify the serotypes. Genome patterns were determined by pulsed field gel electrophoresis (PFGE). Serotypes A, B, C, D, E, F, G...
متن کاملPulsed-field gel electrophoresis of very large DNA molecules.
FACTORS THAT AFFECT PFG SEPARATIONS........................................................................ 294 Molecular Properties ............................................... .................................... ............. 294 Pulse Times............................................................................................................... 295 Electrical Field Shape ..............
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ژورنال
عنوان ژورنال: SEIBUTSU BUTSURI KAGAKU
سال: 1991
ISSN: 0031-9082,1349-9785
DOI: 10.2198/sbk.35.107